The section of the Main Yarra Trail beside the Melbourne Convention and Exhibition Centre will be closed from 4pm to 9pm on Sunday 4 February.
MCEC is hosting a a convention event in the Ferris Wheel area near the trail.
A detour will be in place and bike riders will be able to continue beside the Yarra, using the boardwalk along the river's edge in Polly Woodside Park.
Traffic management will be in place.
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